DNA UV Damage-related Control of Ribonucleotide Reductase; PossibleRelationship to Mutagenesis and the Incidence of and Attitudes toward Skin Cancer in Several Western Nations
| Title | DNA UV Damage-related Control of Ribonucleotide Reductase; PossibleRelationship to Mutagenesis and the Incidence of and Attitudes toward Skin Cancer in Several Western Nations |
| Publication Type | Thesis |
| Year of Publication | 2006 |
| Authors | Stenberg, John C. |
| Academic Department | Biochemistry and Biophysics |
| Thesis Advisor | Mathews, Dr. Christopher K. |
| Degree | Honors Baccalaureate of Arts in International Studies in Biochemistry and Biophysics |
| Number of Pages | 30 |
| Date Published | 06/2006 |
| University | Oregon State University |
| City | Corvallis |
| Thesis Type | Undergraduate |
| Keywords | DNA mutation, Error-prone SOS repair response, Malignant melanoma, Ribonucleotide (rNDP) reductase, skin cancer, Squamous / Basal cell carcinoma, ultravioloet |
| Abstract | Two strains of Escherichia coli were studied to determine the effect of constitutive expression of the SOS DNA damage repair response on the activity of ribonucleotide reductase. Activity of the reductase and cellular deoxynucleoside triphosphate (dNTP) pools were both determined before and after exposure of the cells to ultraviolet radiation. The experiments showed no significant difference in enzyme activity or cellular dNTP pool levels between normal E. coli and cells constitutively expressing the SOS DNA damage repair response. Although the SOS response and proportional accumulation of dNTPs both lead to DNA mutation, the occurrence of the latter after a damage event is not controlled or promoted by the first. |
