Suppressor of Thioredoxin Lethality Gene (stl1) Determination in Yeast
Presented by: Alicia Smith
Mentor: Gary Merrill
Department of Biochemistry & Biophysics, Oregon State University
Thioredoxin reductase is a non-essential gene involved in redox regulation of p53. Deletion of the TRR1 gene encoding thioredoxin reductase in yeast results in poor viability and slow growth of cells. Results of experiments incorporating selective mating, sporulation, and tetrad dissection may indicate the presence of a suppressor mutation that must be co-inherited with the trr1 allele in order for a spore to be viable.