URSA Engage Program 2013
Position Descriptions for Students to Apply For
Faculty Name & Department/School: Dr. Michael Freitag, Biochemistry/Biophysics
Research Project Title: Regulation of Secondary Metabolite Expression by Chromatin Structure
Large gene clusters in filamentous fungi are regulated by the underlying chromatin structure, a combination of DNA, proteins and RNA. We wish to determine how genes within secondary metabolite clusters are silenced or expressed as a consequence of posttranslational modifications on histone proteins. To this end we generate mutants, make gene deletion constructs and carry out protein complex isolations. The long-term goal is to generate a regulatory network that explains how large chromatin regions are coordinately silenced or expressed.
What skills will students obtain in this project?
Introduction to current molecular biology (PCR, cloning, site-directed mutagenesis, transformation, gene-targeting), biochemical (SDS-PAGE, protein purification by affinity chromatography) and cytological (microscopy with fluorescently labeled proteins) techniques.
Introduction to fungal genetics (genotyping and phenotyping, haploid Mendelian genetics).
Introduction to high-throughput sequencing methods (library construction and data analyses).
Student research tasks:
The successfully candidate will assist with generation of constructs to decipher the control and function of histone H3 methylation.
Specific responsibilities include:
Upkeep of laboratory stocks (media, buffers).
Genotyping of strains by PCR
Generation of deletion mutants or tagged strains by transformation
Maintenance of strains
Strain database management
attention to detail
good organizational skills
good inter-personal skills
biochemistry or microbiology students
Period of work - Spring and Fall
Number of hours per week expected of student: 10-15 hrs in three large blocks
Preferred Faculty Contact Information: email: email@example.com
(note - applications are NOT sent to mentors - see instructions)
q Yes X No: Human Subjects
q Yes X No: Live Vertebrate Animals
X Yes q No: Recombinant DNA Molecules Transgenic Plants or Animal, and/or Infectious Agents of Plants, Animals, or Humans
q Yes X No: Ionizing Radiation
X Yes q No: Chemical Carcinogens
q Yes X No: Scuba Diving, Free Diving
q Yes X No: Small Boating Operations
Approval Number(s) from above, if relevant ________IBC #0912__________
If you are interested in applying for this position, you MUST complete the student application HERE and email it to Dr. Kevin Ahern at firstname.lastname@example.org
Do NOT email your application to the faculty mentor.