1. Samples submitted in latex gloves or OB sleeves, or other inappropriate packaging, will not be processed or tested.
2. All test require that strict sample hygiene is used to prevent any cross-contamination between cases.
3. Collect necessary samples. Use a Ziplock bag or Whirlpak bag (no OB sleeves) for each fresh tissues that is collected. DO NOT POOL TISSUES. Clearly label the bag with the name of the owner and the specimen contained with the bag.
4. Collect samples for histopathology. Minimal sampling should include rumen, abomasum, duodenum, jejunum, ileum, colon and any gross lesions of the gastrointestinal tract. Samples should be opened along the mesenteric border to expose the lumen and placed in 10% formalin. Samples of liver, kidney, lung, spleen, and cardiac muscle should also be included in 10% formalin.
5. Collect samples for virology. Ileal contents/scrapings for direct EM exam is preferred (placed in a plastic vial). If BVDV is suspected then additional fresh samples such as distal ileum, mesenteric lymph node, and thymus may be submitted for PCR and/or VI (at additional cost).
6. Collect samples for bacteriology. Samples should include a fresh segment of colon, mesenteric lymph node, and any grossly abnormal tissue. Fresh samples of liver, lung, and kidney should also be included. Aerobic culture will be performed. NOTE: Submit all fresh tissues individually in either a Whirlpak bag or Ziplock bag.
7. Collect a sample for parasitology. Fecal material should be collected in a plastic vial for subsequent Cryptosporidium exam.
8. Follow shipping instructions as per section entitled, Patient Specimen Shipping Instructions.