Design, Synthesis and Application of a Hydrazide-Functionalized Isotope-Coded Affinity Tag (HICAT) for the Quantification of Oxylipid-Protein Conjugates

 

Reference ID:  06-20

Inventors:       Jan Frederik Stevens, Claudia Maier

 

Description:    Current techniques for detecting lipid- generated aldehydes have the important disadvantage that they are not quantitative. This invention however provides a new analytical method that is capable of identifying, characterizing, and quantifying oxylipids-conjugated proteins in complex peptide mixtures.

The invention provides a new group of reagents, Hydrazide-Functionalized Isotope-Coded Affinity Tag (HICAT), which include a biotin affinity moiety, a linker whiith either carbon-12 or carbon-13 and aldehyde-reactive moiety (either hydrazide or hydroxylamine). These two groups react with aldehydes arising from the adduction of lipid-derived 2-alkenals with proteins and this methodology allows the affinity enrichment of biotinylated oxylipid peptide conjugates and enables their recognition by mass spectrometry.

 

Applications:  Immunology

                      Pharmaceutical sciences

                      BioProduction

 

License Terms: Exclusive or non-exclusive

 

Patent Status:  US patent pending

For more information, contact:

Mitra Kiani, Licensing Associate
Technology Transfer Office
Oregon State University
308 Kerr Administration Building
Corvallis, Oregon 97331-2140, USA

E-mail: Mitra.Kiani@oregonstate.edu
Telephone: (541) 737-3030
Facsimile: (541) 737-3093