CSS 322, Principles of Potato Production

A. Mosley, I. Vales, J. McMorran, S. Yilma

CS 421; 737-5835; alvin.r.mosley@oregonstate.edu

MWF 9:00 - 9:50 in CS 122; T 12-12:50 in CS 150

Discussion Topics By Class Session

  1. Introductory remarks; description of the potato – botany & taxonomy
  2. Botany, taxonomy continued; crop origin and history
  3. History; importance; Oregon, U.S., international production; propagation
  4. “Seed” production; certification; Limited-Generation scheme; breeding and
    varieties
  5. Varieties, continued – fresh, chipping, frozen processing, specialty; roles of starch, sugar in processing
  6. Field preparation; tillage, fertilization, herbicides, systemic insecticides, etc
  7. Preparing seed, planting, concurrent operations – herbicides, insecticides for
    wireworms, etc.
  8. Planting to emergence; emergence to “layby”
  9. Layby to vine kill; harvest
  10. Storage -- ventilation, recirculation, temperature, humidity, other.
  11. Fertilization and irrigation, in-depth
  12. Irrigation, continued; pest management – diseases, insects, weeds
  13. Laboratory and greenhouse tour; tissue culture, winter growouts; eye-indexing,
    etc. S. Yilma and J. McMorran
  14. Final exam, CS 122. See practice exams at the bottom of this document

 

1. Organizational

-Introductions -- students, instructors, guests

--Purpose

Much of the informattion covered in this class can be found on websites at http://oregonstate.edu/potatoes (The Potato Information Exchange, or PIE) and http://cropandsoil.oregonstate.edu/classes/CSS322/Cppina.htm -- Commercial Potato Production in North America published by the Potato Association of America, PAA). Rough class notes are located at http://oregonstate.edu/potatoes/CSS322WebNotes.html.

Three practice exams are included at the end of these notes. Please observe that exams typically involve an essay based on 10 questions selected from a dozen or so. If you answer more than 10, your 10 best responses will be used to calculate your class rank and grade.

CSS 322 Timeline -- MWF, 9 – 9:50 in CS 122 and T, 12-12:50 in CS 150

 

2. Botany, Taxonomy, Origin

-The White or “Irish” potato is typically thought of as Solanum tuberosum L.

Solanum tuberosum is tuber-bearing tetraploid species belonging to the Solanaceae (nightshade) family. The cultivated potato has 4 sets of chromosomes; however, there are approximately 150 species of "potatoes" or solanums which are tuber-bearing tetraploids, triploids, diploids, etc.

 

--The center of origin for potatoes is thought to be in the Andes of Peru, Chile, Boliva, Ecuador, etc. but species occur over a broad geographical area including even portions of the southwestern U.S.; wild species are mostly short-day plants for

flowering and tuberizing (many wild species originating near the Equator will not tuberize in Oregon).

 

--Most species are found in S. American highlands, above several thousand feet; some grow at 15,000 +

--Many scientists contend that potatoes originated in Mexico and moved southward while others assume the opposite.

--At least two potato species are native to the S.W. U.S. -- Solanum jamesii (U.S.

only) and Solanum fendleri (U.S., Mexico, Columbia – suggesting 

intercontinental movement?)

--Germplasm banks collect, study, preserve and distribute wild species from multiple locations. The U.S. potato germplasm bank is located at Sturgeon Bay, Wisconsin (http://www.ars-grin.gov/ars/MidWest/NR6/index.html#AU). Other collections are maintained at the International Potato Center in Lima, Peru, the Vavilov Institute in Russia and at various German, Dutch and Polish sites (See http://www.cgn.wageningen-ur.nl/pgr/collections/crops/potato/genbanks.htm for further details and a large listing).

 

--Close relatives of potato include tomato, tobacco, petunia, pepper, eggplant, jimson weed (loco weed), deadly nightshade (Belladonna).

 

--Many Solanum species contain poisonous alkaloids (potato = solanine, chaconine; tomato – tomatine; tobacco = nicotine (a good greenhouse insecticide); belladonna = atropine. Atropine is used to inhibit the actions of acetylcholine -- prevents actions of smooth muscles, used to dilate eyes, etc. Alkaloids are produced in the roots and typically translocated throughout the plant.

 

--Most Solanums are graft compatible; examples--tomato scion on potato rootstock = "pomato"; tobacco on tomato or potato rootstocks will be nicotine-free because alkaloids are produced in roots; without tobacco roots, no nicotine in tops. Similarly, hop plants grafted onto marijuana (hemp) rootstocks will contain various attractive foliar alkaloids and can be smoked (not recommended!).

 

Pomatoes, anyone?? Or nicotine-free tobacco?

 

--The potato is an annual dicot (two seed leaves as opposed to grasses, etc.) when grown from botanical seeds, but is treated as a perennial because of commercial regrowth/propagation from tubers; the tuber is not only the chief means for potato propagation, but also the primary storage organ and major human food source.

 

 

 

 

--The potato tuber is a modified stem:

  1. about 70-75% water, 25-30% dry matter; 
  2. contains high levels of starch (20% more or less);
  3. contains some sugars (<3%?);
  4. 5-8% protein on a dry wt basis;
  5. potato protein is almost perfect—complete set of amino acids, etc;
  6. good source of some vitamins & minerals; 
  7. only about 90 calories/average tuber when baked or boiled.

 

--Potato vs. wheat protein:

The potato is a good source of protein; for example, in Oregon

potato – (5% protein x 20% d.m. x 21 tons/acre = 400 lbs./acre of protein

(complete, equivalent to egg)

wheat (10% protein x 63 bu/acre x 60 lbs = 373 lbs/acre of protein (some amino

acids limiting)

-- Potato Nutritional Value

Note: A diet of milk and potatoes is relatively complete but might get boring. Michigan State University studies demonstrated that graduate students can survive indefinitely on a diet of potatoes and beer(?).

Apparently an Adult Irishman of the mid 1800’s ate up to 14 lbs/day. The potato tuber is a fairly good source of potassium and Vit. C and was sometimes used to prevent  scurvy by old-time sailors.

3.History

See Also http://collections.ic.gc.ca/Potato/histtimeline/index.asp

 

--Cultivated in Andes for 13,000 years!?

 

--Native peoples (Incas, etc.) freeze-dried potatoes at least 3-5,000 years ago by  freezing, thawing and squeezing juices out; product was called Chuno, with accent over the n.

 

--1500’s to Mediterranean Europe by conquistadores?

 

--1600’s Europe proper

 

--1600 – 1800, development of varieties in British Isles—Ireland

 

--1845 Irish Famine, 2 million dead or emigrated to N. America; typical adult Irish male ate 12-14 lbs/day; no other food sources because grains, other commodities were used to pay rent to English landlords; late blight appeared and was uncontrollable, killed vines, rotted tubers in field and storage; fungus confused with “bad air”, divine curses -- see:

 

 

 

 

--1700-1800’s, white fleshed varieties in North America

 

--1900’s, Pacific Rim and India

4.Importance

 

--4th leading world crop after wheat, rice, corn; fastest growing of top 4.

 

--greatest production of protein/food per acre of all major crops

 

--potato is most important in north temperate regions despite short-day origins

primarily in N. temperate zone, but also in Australia, New Zealand, S. America

Leading Potato Producers, Millions of Metric Tons

 
1996
2002
U.S.
18
21
Russia
36
32
China
35
65
Poland
33.6
15.4
Ukraine
21
16.1
India
15.5
24

 

Total U.S. Potato Exports, 2001
 
!,000 Lbs., Product Weight
$1,000
Fresh
693,195
122,297
Frozen Fries
1,051,670
342,007
Other Frozen
49,937
22153
Chips
163,835
165,945
Flakes & Granules
64,895
32,082
Dried
6,543
3,147
Other
58,458
35,686
Total
2,088,533
723,316

 

2002 U.S. Production – 3.15 $billion, farm gate; about 5.5 $billion in finished (processed) form

 

Farm Gate$ , 2002

Idaho,720 $million; Washington, 514;California, 307, Wisconsin, 221; Oregon, 134

Oregon processed potato value = $300 - $350 million

Oregon potato personnel payroll = ~ $60 million, more than all other agronomic crops combined

Approximate Oregon acres – Columbia Basin, 26,000; Klamath, 12,000; Malheur, 10,000, Central Oregon, 3000; Willamette Valley, 1200; notheastern Oregon, 600 seed

 

Oregon crops, 2001: Farm gate, $millions – All hay, 333; all grass seed, 324; potatoes, 132; all wheat, 106; onions, 74; pears, 63 

finished/processed – potatoes = $300 million.

5.Propagation (for commercial production)

--Potatoes are cloned commercially

 

--Implications of cloning:

1.cloning assures genetic purity, uniformity of product (fries, chips, etc.) except for rare mutations

2.cloning increases disease spread, especially viruses (Y, X, A, M, PLRV, etc.), bacteria, fungi; examples, Bacterial Ring Rot, Late Blight

3.cloning favors high yields relative to true potato seeds  

--Seed Potato Production -- potato seed tuber production is highly technical with many built in procedural and quality assurance standards. At this time virtually all North American seed potatoes are derived from meristems (located in the heart of buds) via test tubes and greenhouses.

An excised meristem is typically 0.2 - 0.5 mm in diameter, or approximately half the diameter of a narrow pencil lead. The meristem is the meristematic center of a bud; meristematic cells divide to give rise to all other tissues. Meristems have no vascular system (several intermediate cells between vascular tisse and meristematic cells), so are less prone to viral, fungal and bacterial infection via the vascular system. All buds (apical, lateral, sprout) have meristems and can be used for seed increase purposes.

Meristems are placed on a nutrient-rich agar in test tubes and grown into plantlets.

Plantlets such as the one on the right above can be multiplied by 4 or 5x monthly as needed by dividing stems into "nodal cuttings" which are then rooted in either other test tubes or greenhouse potting medium depending on program needs. Nodes denote the locations of leaf/bud junctions on plant stems; they are frequently slightly swollen compared to the rest of the stem. Disease-free, certified Pre-nuclear minitubers are produced from rooted "nodal" cuttings in greenhouses. Cuttings without nodes obviously will not produce plants.

Because seed potatoes rapidly pick up viruses and other diseases in the field, all seed-producing states have adopted "limited-generation" seed production/certification schemes. Note that generations typically drop one number per year. However, disease levels above certification tolerances can cause seed lots to drop by several generations in a single season. Certification tolerances for potato seed diseases are typically very low (see http://www.oscs.orst.edu/potato/index.shtml). Note that the generational flow is always downward; that is, a specific generation of seed can only be produced from an earlier, more disease-free generation.

Typical Limited-Generation Seed Production Scheme

Class of Seed Planted
Class Produced
Prenuclear (Lab & Greenhouse)
Nuclear
Nuclear
G1 (Generation 1)
G1
G2
G2
G3
G3
G4
G4
G5

Some guiding principles for producing healthy seed potatoes: 

Problems—certification programs vary from state to state in standards/tolerances, quality of personnel, terminology, and reputation.

Certification Methods:

 

Certification inspectors typically inspect potato fields at least twice during the growing season. The first inspection tends to concentrate on viruses, off-types, blackleg (bacterial infection) and other diseases which show symptoms early. The second inspection covers the preceding as well as Bacterial Ring Rot, Late Blight and other late-occurring problems. Because of its devastating effects, there is an absolute zero tolerance for BRR in all certification programs. The zero tolerance is also in effect for other problems such as certain nematodes, tuber moth and powdery scab.

Certification inspectors typically inspect fields from one corner diagonally across the rows because some problems (BRR, for example) may be expressed row by row. The inspector assumes that he is viewing a certain number of plants in each row. By counting the number of problem plants and rows, he/she can fairly accurately estimate the number of problem plants. If the percentages exceed certification tolerances, the field is rejected.

Because field inspection is by necessity a visual process, some plants may be questionable and call for laboratory verification by ELISA, Latex Agglutination, Electron Microscopy or other methods. Most Limited-Generation schemes require laboratory testing for PVX through the first one or two field generations.

The second field inspection typically occurs well before plants have died. Therefore, the potential for undetected late-season infection is high. For this reason virtually all certification programs require some form of winter grow-out testing. For winter tests, a percentage of the tubers (usually 400 or so in Oregon) for each seed lot are planted and the resultant plants are visually examined for disease symptoms and herbicide injury. As in field inspections, questionable plants and all plants of varieties which do not express PVY symptoms are lab tested.

Because of all these constraints, seed potato production is a “white knuckle ride” and growers never know whether their crop will be certified until late in the winter in most instances.

Breeding and eye-indexing:

Because breeding clones (selections) must be kept healthy for further increase and testing, breeders go to extraordinary means to prevent disease buildup. These means include electron microscopy and a number of sophisticated serological tests including Enzyme-Linked Immunosorbent Antibody, Latex Agglutination and numerous others. 

One traditional tool used by all breeders, and perhaps the most effective, is “eye-indexing”.Eye indexing typically begins with single- or five-hill selections and continues throughout the remaining 10 or 12 years leading to release. Eye indexing involves removing an eye from a tuber using a melon scoop, numbering the eye and the mother tuber identically, and then planting the eye to produce a plant for symptom evaluation. Grow-out plants are evaluated for viruses and other problems when 8-12 inches tall. Those with obvious problems are noted and the corresponding tubers are discarded. Only tubers of healthy appearing plants are kept for replanting and increase. Eye-indexing is an essential component of most breeding programs because of the large numbers of progeny involved and severe virus pressure.

 

For more information on seed production, see http://cropandsoil.oregonstate.edu/fpsp/index.html; http://www.oscs.orst.edu/potato/index.shtml; and http://oregonstate.edu/potatoes/potliv.html#Seed

 

6. Variety Selection  

 

 

 

(see http://oregonstate.edu/potatoes/variety.htm for additional information and images of varieties)

 

Potato breeding typically proceeds as follows: Male x female crosses ® berries & seeds ® seedling transplants ® plants (clones) in pots ® tubers ® single-hill field trials (2% saved) ® 5 hill trials (5% saved), etc. ®etc. for 12 more years until the clone is named and released. These fairly typical steps are illustrated in the Oregon flowchart below.

 

Transferring pollen from one plant to another produces seeds (TPS) which can be grown into plants producing tubers.

Tubers can be propagated ad infinitum by division into seedpieces.

 

 

Considerations for selecting varieties

Many varieties and types are available to commercial potato growers; potato tubers come in many color combinations! Varietal selection at the commercial level is ultimately determined by the profit motive. Commercial growers will only use varieties they expect to be marketable and profitable. Home gardeners have more leeway.

 

The following are important varietal selection criteria if profits are to be maximized:

 

1.Intended use – frozen processing, chips, starch, fresh market (table) use, roadside marketing, etc.;

2.Color, shape, season of maturity, storage dormancy, yield, grade-out;

3.Resistance to diseases, pests and disorders; and 

4.Adaptability to local growing conditions

5.Express symptoms when pathogens are present

 

 

Common processing varieties include

Specific gravity is an indicator of potato density, dry matter and starch content. Starch content strongly affects potato usage as shown in the table below.

 Specific Gravity and Dry Matter Content Vs. Potato Usage*

Specific Gravity
% Starch
Texture
Common Usage
<1.060 (v. low)
<16.2
V. Soggy
Salads, pany fry, can, boil
1.060-1.069 (low)
16.8-18.1
Soggy
Pan fry, salads, boil, can
1.070-1.079 (medium)
18.2-20.2
Waxy
Boil, chips, fries, may slough when boiled
1.080-1.089 (high)
20.3-22.3
Mealy, dry
Bake, chips, fries, some varieties slough when boiled
>1.089 (v. high)
>22.3
Very mealy, dry
Bake, fries, chips; tendency to produce brittle chips and slough when boiled

*starch accounts for 60-80% of potato total dry matter.

Starch, why is it important to processors of fries, chips, flakes and granules?

1.Higher starch means less water in tuber tissues;

2.Water is displaced by fat/oil during frying, so high water/low starch potatoes soak up more oil;

3.Oily fries, chips are soggy and unappetizing and often dark in color;

4.Oil is expensive;

5. High starch favors high product yield; for example, 

20% dry matter potatoes (high starch) will theoretically produce more pounds of chips/ton of raw product than 15% (low) starch potatoes while also requiring less oil.

Specific gravity, as shown in the table above, is a direct indicator of dry matter content of potatoes and, indirectly, starch content. The relationships are not exact and can be influenced by a number of factors including water and tuber temperatures, wetness of the tubers, etc. The specific gravity of pure water is 1.000, so potatoes are heavier than water and will sink unless the water is amended with starch, sugar or other materials to increase the specific gravity of the solution. Specific gravities are often determined by weighing a sample of potatoes in water and then in air, according to the following formula:

 

Specific Gravity = weight in air/weight in air-weight in water

A potato hydrometer produced by gluing a long, sealed, indexed tube to a float from which a basket is hung is also used for determining specific gravity. The hydrometer requires that an exact weight of potatoes be placed in the basket so that specific gravity can be read directly from the index markings on the tube. The weight in air/weight in water method is typically preferred.

Sugar, why is it important to processors?

 

1.High reducing (6-carbon or hexose) sugars cause dark-colored fried products, especially chips and French fries. Reducing sugars reduce copper in some obscure chemical reaction.In potato, reducing sugars are predominantly the 6-carbon sugars glucose and fructose, which result from the breakdown of starch from tuber reserves or the breakdown of sucrose transported from the plant leaves to the tubers. Sugars, the plant’s energy currency, are stored in tubers as starch. Starch is basically a long-chain polymer of sugar molecules. The relationship between photosynthesis and tuber carbohydrate can be crudely described as follows:

·Photosynthesis > glucose/fructose in leaves > sucrose in phloem transport system > glucose/fructose at action site(s) for respiration and/or storage as starch (a long, endless polymer of sugars) in tubers; starch is therefore a reserve energy currency.

 

Principle: for processing, we want high starch and low sugars almost always.

 

 

Fresh market varieties accent appearance, but yield, storage life and resistances are also important. Taste has been largely ignored in most U.S. potato breeding programs but is slowly resuming its former importance. Most fresh market varieties are relatively low in starch and high in sugar compared to processing varieties. They are, therefore, typically moist-fleshed and fine-textured.

 

Fresh market varieties can be any of many shapes and colors

1.Round reds (red skin, white flesh) – Norland, Pontiac, Redsen, Winema, Mazama, Modoc, Bison, Viking, Red LaSoda

2.Long russets – Russet Norkotah, Century Russet, Klamath Russet, Russet Burbank, Russet Legend; Gem Russet,Klamath Russet

3.Round whites – Superior, Katahdin, Kennebec, Sebago

4.Specialty/gourmet types—red skin/yellow flesh (Desiree); purple skin, flesh (All-blue); yellow flesh (Yukon Gold, Bintje); fingerlings (LaRatte, Yellow Banana, Yellow Finn, Peanut) and so on. See http://oregonstate.edu/potatoes/variety.htm#OtherVarieties for additional listings, descriptions and pictures.

 

 

7. Planting Site Selection and Soil Preparation

·Soil type – As with most crops, potatoes appreciate a well-drained, coarse-textured soil--but in a very big way! A loose, well-drained loamy or sandy soil:

1.Favors a long season (early planting/late harvest);

2.Is more workable and resists crusting from rain or irrigation;

3.Maximizes yield and quality (yield is somewhat proportional to the length of the growing season; for example yields in the short-season Willamette Valley and Central Oregon average about 20 tons/acre while the Hermiston area produces 25-30;

4.Improves aeration which reduces seed piece and tuber decay; and

Note: Erwinia softrot bacteria prefer anaerobic (oxygen-free) conditions associated with wet, crusted or poorly drained soils; under wet conditions, bacteria easily move in free water and invade the tuber via wounds (from fungi such late blight or Fusarium sp., pinkroot,insects, etc.) and lenticels (substomatal cavities) in tuber skins. Once inside the tuber, bacteria multiply rapidly and decay seedpieces and/or tubers. Free moisture and bacteria are a lethal combination for potatoes either in the field or in storage.

A lenticel is a substomatal cavity (remember, the tuber is a modified herbaceous stem). Stomates are ruptured as the tuber skin expands during growth so that guard cells, etc., are displaced and only the cavity truly remains. Lenticels become whitish and very prominent in the presence of excess moisture. Enlarged, infected lenticels are a good indicator of wet conditions.

 

5.Reduces tuber malformations caused by mechanical resistance to expansion. Rocks can be especially deforming.

6.Improves fertilizer and water use efficiency

·Soil Preparation:

1.Potatoes do not require a fine seedbed as with grains and most seeded crops, so don’t overdo it! In fact, potatoes suffer from excess preplant tillage because of potential soil crusting and associated anaerobic (low oxygen) conditions which favor seed piece decay and stand loss.

2.Never irrigate prior to emergence unless a drought situation exists! If the soil is excessively dry, irrigate well ahead of time and plant when the soil is still moist but not wet.

Example: in 1998, the OSU Seed Certification crew over-watered the greenhouse between eye-index plantings and emergence and successfully destroyed (rotted) 90% of the eye index seed pieces! In contrast, under normal preemergence irrigation in 1999, more than 90% of the plants survived.

 

What is eye-indexing? Remember the explanation from earlier?

 

8. Preparing Seed 

North American producers mostly plant seedpieces cut from large, whole tubers.

 

 

Seed tubers should always be warm (>50F) and dry when cut. Warm tubers, especially those whose "eyes" are just beginning to “peep” (that is, with buds or eyes just beginning to enlarge), are physiologically active and begin to heal and emerge rapidly after planting.

Seed pieces should ideally be cut, treated with a suitable fungicide and planted immediately after under good field conditions. Good field conditions imply warm (>45F) and moist but not wet soils. If seed can not be planted immediately because of scheduling or weather complications, special storage precautions (55-60F, high humidity, good ventilation, shallow piles) should be taken to prevent overheating and disease spread and promote rapid healing.

·Cutting causes problems!. Most U.S. seed tubers are large and require cutting; however, some growers, especially in the midwest where seed piece decay is a severe problem, prefer small whole seed tubers called single drops. Because the tubers are not cut, single-drop seed is highly resistant to decay. Most of Europe and the rest of the world use whole seed.

Cutting is a very effective way to spread most potato diseases including some viruses and most bacteria and fungi. Therefore, strict sanitation should be observed at all times, but especially between seed lots. If the seed lot has an obvious disease problem, discarding the seed is a legitimate option. Local potato extension people or other experts should be consulted if serious disease levels are suspected.

Cutters and handling equipment should be thoroughly cleaned and sanitized between lots to prevent spreading diseases from one seed lot to another. For example, if a seed lot has Bacterial Ring Rot, a highly infectious disease, it is vital that the source of the BRR be tied to a specific lot/grower. Seed growers almost invariably pay for ring rot problems provided the commercial grower can provide proof of guilt. If BRR, or other serious seed quality problems are suspected, look for an independent third party inspection and unbiased “expert” witnesses for corroboration. Florida studies from a few years ago demonstrated that a 1% BRR seed infection could result in 100% infection of cut seedpiecs due to contamination and spread throught the cutting equipment.

A seed cutter’s checklist:

1. Warm seed to 50F+ for a few days before cutting to prevent tissue tearing, wake the seed up physiologically, and get it ready for rapid healing and early emergence. Sprouts should just be “peeping” when seed is cut.

2.Seed is mechanically cut at the rate of 1 to several tons/hour/cutter.

3.Keep cutter blades sharp and clean; a sharp blade does less tissue damage.

4.Keep a close eye on the seed being cut—diseases, varietal type, mechanical condition, excess debris, freezing or heat injury, etc.

 

5. Keep a record of planting sequences and where seed lots are located in fields for insurance, legal purposes.

 

6. Always sanitize between lots to prevent disease spread between lots.

·Seed Treatment: Apply fungicides to cut seed pieces immediately after cutting while surfaces are still moist enough for powder to adhere; fungicides are also sometimes applied as liquids either as a spray or dip. Liquids may cause bacterial problems in some situations because of the water involved, but typically not if the seed is handled properly.

Common seed piece fungicides include – Captan, Dithane, Manzate, Maxim, Tops; most are applied as a dust at the rate of 0.5 – 1.0 lbs/100 lbs. of cut seed and can be very beneficial, especially under less than perfect planting/pre-emergent field conditions. Labels for these and other agrichemicals can be found at http://www.greenbook.net or http://www.cdms.net. Other materials such as straight Douglas Fir Bark or talc are sometimes used with success. Douglas Fir bark may act as a simple drying agent to prevent anaerobism and movement of bacteria, but it is thought to have fungicidal properties as well?

·Precutting???

In general growers prefer to cut, treat and plant if conditions are good (moist but not wet soil > 45F). Some growers pre cut seed up to a month early, but Pre- cutting is dangerous because of possible decay.

 

9.Planting

 

 

·How do we plant? Potatoes are planted mechanically at 2-4 mph and from 2 to 6 rows at a time. Fertilizer and other materials such as systemic insecticides are often applied simultaneously depending on local conditions and customs.

·When do we plant? February through July depending on location,