The reassociation kinetics of this eukaryotic DNA show three types of components (indicated by the shaded areas). The arrows identify the Cot1/2 values for each component. In the calculation of the complexity, E. coli DNA was assumed to have a Cot1/2 = 4. Thus the compexity of the slow component (unique DNA with a repetition frequency of 1) = (0.45) (630) (4.2 x 106 bp)/4 = 2.98 x 108 bp. The total size of the genome = 2.98 x 108 bp/0.45 = 6.6 x 108 bp.