Biotechnology Techniques

Purification and Detection

• Separation - HERE
• Detection by UV light of stained DNA or radioactive labeling

Restriction Endonucleases

• Cutting - HERE
• Restriction/Modification - HERE
• Cleavage Sites - HERE

"Cloning"

• Making Recombinant DNA - HERE
• Virus Cloning
• Cell Cloning - HERE
• Plasmids
  • pET Expression Vectors - HERE
  • pBR 322 HERE
  • pUC19 - HERE
• Multiple Cloning Site - HERE
• Inserting DNA Fragments into a Virus HERE
• Selecting for Recombinant DNAs with a Plasmid - HERE
• Blue-White Screening - HERE

Genetic Engineering

• Mutant Mosquitoes
• Transgenic mustard, tomatoes, crops
• Insulin Synthesis in engineered bacteria/yeast - HERE
• Histidine Tagging - HERE
• Making a DNA Library - HERE
• Making cDNA - HERE
• PCR - HERE
• Southern Blotting
• DNA Fingerprinting - HERE
• RFLP Analysis
• Paternity Testing - HERE

DNA Sequencing

• Dideoxynucleotides - HERE
• Sequence Determination - HERE and HERE
• RNA Interference - HERE and HERE

Genomics / Proteomics

• Microarrays - HERE
• Expression Profiles tell relative gene expression in a treated cell compared to a control