Protein Purification and Characterization

Methods

• Scheme - HERE
• Centrifugation - HERE
• Column Chromatography -
  • Gel Filtration - HERE
  • Affinity Chromatography - HERE
  • Ion-Exchange - HERE / HERE / HERE
• Electrophoresis - HERE / HERE
  • Agarose - HERE
  • Acrylamide - HERE
  • Two-dimensional - HERE
• HPLC - HERE
• Protein Digestion
  • Trypsin - HERE
  • Chymotrypsin - HERE
  • Cyanogen Bromide - HERE
  • Overlap Analysis - HERE