Catalytic Strategies Outline

Proteases

• Hydrolysis reaction HERE
• Specificity of chymotrypsin cleavage HERE
• Chymotrypsin's unusually reactive serine HERE
• Chromogenic substrate HERE
• Kinetics of action HERE
• Covalent intermediate HERE
• Catalytic triad HERE
• Catalytic mechanism HERE - Steps HERE / HERE / HERE / HERE / HERE / HERE / HERE / HERE
• Oxyanion hole HERE / Hydrophobic pocket HERE
• Trypsin-chymotrypsin similarity HERE
• S1 pockets of chymotrypsin, trypsin, and elastase HERE
• Effects of site-directed mutagenesis of subtilisin HERE
• Active sites of three classes of proteases HERE / HERE / HERE
• Activation strategies HERE / HERE / HERE

Carbonic Anhydrase

• Carbonic anhydrase reaction HERE / pH effects HERE
• Water-bound zinc HERE
• Carbonic anhydrase mechanism HERE
• Kinetics of water deprotonation HERE / Buffer effects HERE and HERE
• Histidine proton shuttle HERE

Restriction Enzymes

• Phosphodiester bond hydrolysis HERE
• Mechanism I HERE
• Magnesium in EcoRV HERE
• EcoRV recognition site HERE
• Distortion of recognition site HERE
• Methylation of adenine HERE

Myosins

• ATP hydrolysis for motion HERE
• Myosin structure HERE
• Facilitating water attack HERE
• Myosin conformation change HERE
• P-loop enzymes (myosin included) HERE , HERE, and HERE