Protein Characterization Outline

Purification

• Lactate dehydrogenase HERE
• Differential centrifugation HERE
• Dialysis HERE
• Gel filtration HERE
• Ion-exchange chromatography HERE / Supports HERE
• Affinity chromatography HERE
• HPLC HERE
• Agarose Gel Electrophoresis
• Polyacrylamide gel electrophoresis (PAGE) HERE and HERE
• Sodium dodecyl sulfate (SDS) structure HERE
• Mass determination by electrophoresis HERE
• Isoelectric focusing HERE
• Two-dimensional gel electrophoresis HERE and HERE
• Differences - Normal vs Colorectal tumor HERE
• Example purification HERE and HERE

Characterization

• Amino Acid Analysis HERE
  • Fluorescent labels
    • Fluorescamine HERE
    • Ninhydrin HER
• Polypeptide cleavage agents HERE
• Disulfide bond reduction HERE and blocking HERE
• DNA sequencing and protein sequencing HERE
• Immunological Techniques
  • Antibody structure HERE and HERE
  • Antigen-antibody interactions HERE
  • Western blotting HERE
  • Cellular visualization and localization HERE
  • Immunoelectron microscopy HERE
• Mass Spectrometry - MALDI-TOF HERE
• Sequence analysis by MALDI-TOF HERE
• X-ray Crystallography
  • Diffraction HERE and HERE
  • Electron density map HERE
• NMR signals HERE
• NMR basis HERE
• One dimensional NMR HERE and HERE
• Nuclear Overhauser effect HERE
• Detecting short proton-proton distances HERE