Environmental Health & Safety

BioSafety Cabinets

• Certification
• Comparison
• BSC Class I
• BSC Class IIA
• BSC Class IIB1
• BSC Class IIB2
• BSC Class IIB3
• BSC Class III

Fume Hoods

• Certification
• Guidelines
• Types

 

BioSafety Cabinet Class I

A. front opening
B. sash
C. exhaust HEPA
D. exhaust plenum

BioSafety Cabinet Class IIA

A. front opening
B. sash
C. exhaust HEPA filter
D. rear plenum
E. supply HEPA filter
F. blower

BioSafety Cabinet Class IIB1 - classic design

Connection to building exhaust system required.

A. front opening
B. sash
C. exhaust HEPA filter
D. supply HEPA filter
E. negative pressure exhaust plenum
F. blower
G. additional HEPA filter for air supply

Note: The cabinet exhaust needs to be connected to the building exhaust.

BioSafety Cabinet Class IIB1 - benchtop design

Connection to building exhaust system required.

A. front opening
B. sash
C. exhaust HEPA filter
D. supply plenum
E. supply HEPA filter
F. blower
G. negative pressure exhaust plenum

Note: The cabinet exhaust needs to be connected to the building exhaust.

BioSafety Cabinet Class IIB2

Connection to building exhaust system required.

A. front opening
B. sash
C. exhaust HEPA filter
D. supply HEPA filter
E. negative pressure exhaust plenum
F. supply blower G. filter screen

Note:The carbon filter in the building exhaust is not shown. The cabinet exhaust needs to be connected to the building exhaust system.

BioSafety Cabinet Class IIB3

Connection to building exhaust system required.

A. front opening
B. sash
C. exhaust HEPA filter
D. supply HEPA filter
E. positive pressure plenum
F. negative pressure plenum

Note: The cabinet exhaust needs to be connected to the building exhaust system.

BioSafety Cabinet Class III

Connection to building exhaust system required.

A. glove ports with O-ring for attaching arm-length gloves to cabinet
B. sash
C. exhaust HEPA filter
D. supply HEPA filter
E. double-ended autoclave or pass-through box

Note: A chemical dunk tank may be installed which would be located beneath the work surface of the BSC with access from above. The cabinet exhaust needs to be connected to the building exhaust system.