Digestion and Biotransformation of Fatty Acids by Pseudomonas aeruginosa
Speaker: Simeon Andrews
Abstract: Pseudomonas aeruginosa is known to biotransform oleic acid into typical lipoxygenase products. Furthermore, a lipoxygenase gene has been found in this organism, which has been cloned, though not successfully expressed. The intent of the experiment was to characterize this enzymes action.
To do this, fatty acids were added to cultures of PAOI which were 24 hours old. After incubation for another two days, the supernatant was extracted with organic solvents and derivatized. The results were analyzed by gas chromatography/mass spectrometry.
Results show that the lipoxygenase seems specific to monounsaturated fatty acids, as all other fatty acids were only digested, not biotransformed. A curious result has been that elaidic acid was changed into oleic acid, an unusual isomerization.