Purification of Nisin
Speaker: Megan Oest
Abstract:: Nisin is a small bacterially synthesized antimicrobial peptide with many unusual properties, including high levels surface activity and stability at high temperature and low pH. These properties make Nisin ideal for applications in medical and food technology fields. In order to research these applications, however, a pure form of Nisin must be obtained. A lack of commercially purified preparations has motivated the development of a purification method for Nisin that might also be applied to other antimicrobial peptides. Through experimentation with various types of chromatography and other standard protein isolation methods, it was determined that ion-exchange chromatography, combined with filtration, provides an efficient and effective means of purifying and concentrating Nisin. The success of this method rivals that of known commercial preparations.