Isolation of Recombinant T4 Phage Proteins Involved in DNA Precursor Biosynthesis

Speaker: Isaiah Schauer

Abstract: PCR was used to amplify the T4 phage genes gp39 and a truncated form pgp39, giving yields of .17 mg/ml and .25 mg/ml respectively. The expression vector pCYB4 was utilized for recombinant cloning with E.coli cells. To date, screening of the transformant colonies has revealed no gene-recombinant plasmid induction. Work will continue on this project. Once gp39 and pgp39 are overexpressed, they will be analyzed for interaction with nucleoside diphosphokinase to determine positive or negative involvement in the putative dNTP synthetase complex.