Characterization of Mice Lacking the gamma - Glutamyl Transpeptidase
Presented by: Jenny Antonissen
Mentor: Donald Reed
Department of Biochemistry & Biophysics, Oregon State University
My research concerns an antioxidant called glutathione. Glutathione is very abundant in everyone's cells and mitochondria, and is a highly protective mechanism against oxidative stress. Glutathione is recycled in the body with the
help of the enzyme gamma-glutamyl transpeptidase. Dr. Reed's lab has a colony of mice that have this enzyme knocked out. The study of these mice will lead to a greater understanding of the deficiencies that occur when glutathione is not abundant in the body. The question I am asking is how the gluathione levels in the tissues differ between those mice that do and do not have the GGT enzyme. A working hypothesis is that the levels of glutathione in the knockout mice will be much lower.