URSA Engage Program 2013
Position Descriptions for Students to Apply For


Faculty Name & Department/School: Dr. Gary Merrill / Biochemistry & Biophysics


Research Project Title: Genetic Analysis of Carbonyl Reductase Function


Project Overview 
Carbonyl reductases (Cbrs) are proteins that can reduce carbonyls to alcohols.  The in vivo substrates and role of these proteins are unknown.  Yeast have ten Cbrs.  Deletion of any one has no phenotype.  By analyzing combinatorial Cbr deletion mutants in yeast, we will elucidate the role of these proteins.



What skills will students obtain in this project? 
Students will gain experience using mendelian genetics to test hypotheses.  Students will learn how to mate yeast, select diploids, sporulate diploids, and determine the genotype of random spore segregants. Student will learn how to keep a notebook, design experiments, and present their work orally.



Student research tasks
Formulation and pouring of rich and defined medium agar plates; mating and selection of diploids; sporulation; disruption of asci with ether; plating of random spores; picking of random spores to microtiter plates; replicaplating to selective plates to determine inheritance of markers including mating type.



Period of work - Spring and Fall


Number of hours per week expected of student:    Five (This proposal is aimed for one to three students, each at 5 hours per week)


Preferred Faculty Contact Information: merrillg@onid.orst.edu (note - applications are NOT sent to mentors - see instructions)

q Yes    x  No:   Human Subjects 

q Yes    x  No:   Live Vertebrate Animals

q Yes    x  No:   Recombinant DNA Molecules Transgenic Plants or Animal, and/or Infectious Agents of Plants, Animals, or Humans

q Yes    x  No:   Ionizing Radiation

q Yes    x  No:   Chemical Carcinogens

q Yes    x  No:   Scuba Diving, Free Diving

q Yes    x  No:   Small Boating Operations

If you are interested in applying for this position, you MUST complete the student application HERE and email it to Dr. Kevin Ahern at ahernk@onid.orst.edu

Do NOT email your application to the faculty mentor.