URSA Engage Program 2013
Position Descriptions for Students to Apply For
Faculty Name & Department/School: Dr. A. Morrie Craig (Biomedical Sciences) & Jennifer M. Duringer (Environmental & Molecular Toxicology)
Research Project Title: Fate and Metabolism of the Forage Mycotoxin Lolitrem B in Cattle
Perennial ryegrass hay is an important source of forage for a variety of livestock species. Unfortunately, an endophyte fungus called Neotyphodium lolii frequently colonizes this grass which can result in the production of the tremorgenic mycotoxin lolitrem B. The goal of this project will be to evaluate the urine and blood of cattle who were fed varying doses of lolitrem B-containing perennial ryegrass hay for 60 days for lolitrem B and its metabolic breakdown products via LC-MS/MS.
What skills will students obtain in this project?
-Laboratory safety techniques and successful execution of an experiment.
-Proper record keeping including maintaining a lab notebook and timely summaries of progress.
-Analytical chemistry skills including use of quality assurance/quality control principles, solid phase extraction, high performance liquid chromatography and mass spectrometry.
-Ability to use Excel and other software programs to summarize results.
Student research tasks:
-Extract urine and blood samples for lolitrem B and its metabolites using solid phase extraction.
-Process samples using appropriate software for quantity of lolitrem B and its metabolites.
-Summarize data using Excel and other software programs to get basic statistics like average and standard deviation and chart trends graphically.
Period of work - Spring and Fall
Number of hours per week expected of student: 6-10
Preferred Faculty Contact Information: A. Morrie Craig: 541-737-3036, A.Morrie.Craig@oregonstate.edu; Jennifer Duringer: Jennifer.Duringer@oregonstate.edu
(note - applications are NOT sent to mentors - see instructions)
q Yes X No: Human Subjects
q Yes X No: Live Vertebrate Animals
q Yes X No: Recombinant DNA Molecules Transgenic Plants or Animal, and/or Infectious Agents of Plants, Animals, or Humans
q Yes X No: Ionizing Radiation
X Yes q No: Chemical Carcinogens
q Yes X No: Scuba Diving, Free Diving
q Yes X No: Small Boating Operations
Approval Number(s) from above, if relevant: Solvents and some materials used in the extraction procedure are hazardous/carcinogenic. EH&S Laboratory Safety training will be conducted. Feeding trial has already been completed; but since students will be working with animal fluids, Animal Handler training will be conducted before the project begins.
If you are interested in applying for this position, you MUST complete the student application HERE and email it to Dr. Kevin Ahern at email@example.com
Do NOT email your application to the faculty mentor.